Composite

Part:BBa_K609001:Design

Designed by: Ke Liu   Group: iGEM11_HIT-Harbin   (2011-10-01)


acid induced promoter rcfB + repressor lacR of lac operon


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
    Illegal suffix found in sequence at 1112
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal SpeI site found at 1113
    Illegal PstI site found at 1127
    Illegal NotI site found at 15
    Illegal NotI site found at 1120
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
    Illegal BglII site found at 222
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
    Illegal suffix found in sequence at 1113
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal XbaI site found at 24
    Illegal SpeI site found at 1113
    Illegal PstI site found at 1127
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The rcfB promoter is found in Lactococcus lactis IL1403, while the lacR gene is from Lactobacillus. delbrueckii subsp. lactis.


Source

The part is designed to let Lactobacillus bulgaricus stop producing lactic acid when the external pH declines to 5.5 or lower.

References